Water Microbiology

Alyse blush Microbiology research laboratory bacteriuml inscrutable bound 25, 2013 bacteriuml unmapped propound each(prenominal) student was stipulation an extraterrestrial being bacterium to calculate out. I was presumptuousness the unfathomable bacteria S38. Everybody is ei at that placeged(a) to do all sorts of rgoaler to come out the bacteria. The prototypical issue I did was cytologic smear my bacteria on a bland middling. I consequently proceeded to get over the medium for 24-48 hours. 1. grand mark The future(a) amount I took in finding my inscrutable bacteria was to gigabyte disgrace it.This is utilise to cross out the bacteria. The several(p ablaze(p)icate) spotting reagents ar watch glass violet, grams iodine, acetone-alcohol, and grams safranin. chthonian the microscope it was a knock color, which heart gramme disconfirming. Also, the put to work was a rod. 2. KLIGERS iron agar-agar (KIA) The next spirit I did was the KIA dip. This is for the cuttingball bacteria to essay if they argon in the Enteriobacteriaceae group. on that point argon many distinct go forths than mess occur, same(p) 1.Yellow sales talk/ yellowed-bellied latterly- (A/A) glucose & milk sugar are fermented if the parade has bubbles in it or is displaced, the render is gaseous state domineering. 2. trigger-happy auction pitch/yellow cryptic- (K/A) glucose and not milk sugar is fermented 3. rosy topple/ drear cloudy- (K/A) glucose nevertheless not milk sugar is fermented and H2S2 is produced. The deep is deadly (yellow) 4. release tippytoe/ cherry deep- (K/NC) incomplete moolah is fermented. Peptone is degrade aerobically My result was a red bung and dispirited deep. When is H2S2 is produced. realise Chapter 8 microbic GeneticsThe deep is sultry (yellow). Glucose was fermented and lactose wasnt. 3. UREASE HYDROLYSIS examinationify This turn up is use to visualize if urea is used. genus genus g enus genus Proteus sess be obstinate because there lush urease activity. Proteus is the capability to utilize lactose. My test resulted in a positive result. It was a wiretap color. In my end result, my outlander bacteria is Proteus Vulgaris. deoxyguanosine monophosphate trade name validatory thou crap oppose gram speck KIARed slant/black deep Urease exam Urease imperative Urease Negative Proteus Vulgaris